COMPARISON OF MICROFLUIDIC SPERM SORTING (MFSS) VERSUS PHYSIOLOGICAL INTRACYTOPLASMIC SPERM INJECTION (PICSI) VERSUS DENSITY GRADIENT IN HIGH DNA FRAGMENTATION INDEX SPERM SAMPLES

To evaluate the best method for sperm separation in high DNA fragmentation index (DFI) samples. A prospective randomised study was conducted from 1st January 2019 to 2021. Three hundred patients were by computer generated list and divided into 3 groups. Group (n=100) , which processed microfluidic sorter (MFSS) while group B (n=100), selected Physiological Intracytoplasmic Sperm Injection (PICSI) technique C where density gradient technique. Morphologically normal motile injected injection (ICSI) mature oocytes after all three techniques. The period included normozoospermic with (>25% ) oligospermic, asthenozoospermic samples, poor ovarian reserve advanced age excluded study. All grade embryos vitrified transferred frozen embryo replacement cycle. groups compared on basis of fertilisation rate, day development rate clinical pregnancy miscarriage rate. Cycle characteristics (female age, length stimulation, gonadotrophin dose, number embryos) similar among Between groups, there a statistically significant increase observed (Microfluidic Sorting) (60% vs. 42% 38%, p-0.016) (62% 46% 41%, p-0.049), no statistical difference (82% 78% 76%, p-0.80) comparing C. But significantly higher ( 12% 11% 25%, p-0.019) (Group C) We have demonstrated that sorted is not only correlated better integrity but also reproductive outcome. Using it routine practice can help reducing extraneous affect processing techniques achieving sustaining PICSI Density Gradient